A number of abiotic factors such as drought, salinity, extreme temperatures, low or high light intensity, and deficiency or toxic levels of nutrients have huge impacts on crop productivity, and a furthering of our understanding of the molecular, biochemical, and physiological basis of stress tolerance has been widely recognized as critical. InPlant Stress Tolerance: Methods and Protocols, expert researchers cover the most important widely-used techniques, including cutting-edge strategies, in a manner that ensures effective results. Beginning with reviews on dehydration, salinity, and cold tolerance as well as on oxidative stress, the volume then continues with methods involving topics such as describing the identification of stress-regulated genes, proteins, and microRNAs using diverse approaches, measurement of osmotic adjustment, proline levels, enzymes involved in proline metabolism, and sugars as well as determination of ROS levels, lipid peroxidation, ion leakage, and the enzymes involved in ROS detoxification. Written in the highly successfulMethods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date,Plant Stress Tolerance: Methods and Protocolsprovides a wide range of easy-to-follow protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.

Written by leading researchers, this book provides an easily accessible reference for plant stress tolerance. Comprehensive and up-to-date, it provides a wide range of easy-to-follow protocols catering to the needs of those probing this vital area of study.Part I: Reviews and Overview Chapters 1. Dehydration Tolerance in Plants Melvin J. Oliver, John C. Cushman, and Karen L. Koster 2. Approaches to Identifying Genes for Salinity Tolerance, and the Importance of Time Scale Rana Munns 3. Gene Regulation During Cold Stress Acclimation in Plants Viswanathan Chinnusamy, Jian-Kang Zhu, and Ramanjulu Sunkar 4. Redox-Dependent Regulation, Redox Control, and Oxidative Damage in Plant Cells Subjected to Abiotic Stress Karl-Josef Dietz 5. Array Platforms and Bioinformatics Tools for the Analysis of Plant Transcriptome in Response to Abiotic Stress Nese Sreenivasulu, Ramanjulu Sunkar, Ulrich Wobus, and Marc Strickert 6. Metabolic Engineering of Glyoxalase Pathway for Enhancing Stress Tolerance in Plants Ananda Mustafiz, Khirod K. Sahoo, Sneh L. Singla-Pareek, and Sudhir K. Sopory Part II: Genome-Wide Approaches for Identification of Stress Regulated Genes, Proteins, and Small RNAs 7. Genetic Screens to Identify Plant Stress Genes Csaba Papdi, Jeffrey Leung, Mary Prathiba Joseph, Imma Perez Salamó, and László Szabados 8. Arabidopsis Tiling Array Analysis to Identify the Stress-Responsive Genes Akihiro Matsui, Junko Ishida, Taeko Morosawa, Masanori Okamoto, Jong-Myong Kim, Yukio Kurihara, Makiko Kawashima, Maho Tanaka, Taiko Kim To, Kentaro Nakaminami, Eli Kaminuma, Takaho A. Endo, Yoshiki Mochizuki, Shuji Kawaguchi, Norio Kobayashi, Kazuo Shinozaki, Tetsuro Toyoda, and Motoaki Seki 9. Identification of Stress Responsive Genes in Plants Using Suppression Subtraction Hybridization: Ozone Stress as an Example Lila Peal, Michael Puckette, and Ramamurthy Mahalingam 10. Identification of DNA-Binding Proteins and Protein-Protein-Interactions by Yeast One Hybrid and Yeast Two HybridScreening Peter Klein and Karl-Josef Dietz 11. Functional Characterization of Water Deficit Stress-Induced Genes Using RNAi Muthappa Senthil-Kumar, Makarla Udayakumar, and Kirankumar S. Mysore 12. Difference Gel Electrophoresis as a Tool to Discover Stress-Regulated Proteins Jenny Renaut 13. Thiol-Disulfide Redox Proteomics in Plant Research Meenakumari Muthuramalingam, Karl-Josef Dietz, and Elke Ströher 14. Cloning of Stress-Responsive MicroRNAs and other Small RNAs from Plants Jose Luis Reyes, Catalina Arenas-Huertero, and Ramanjulu Sunkar 15. An Array Platform for Identification of Stress-Responsive MicroRNAs in Plants Xiaoyun Jia, Venugopal Mendu, and Guiliang Tang Part III: Antioxidant Enzymes and Metabolites 16. Spectrophotometric Assays for Antioxidant Enzymes in Plants Sathya Elavarthi and Bjorn Martin 17. Affinity Purification and Determination of Enzymatic Activity of Recombinantly Expressed Aldehyde Dehydrogenases Hans-Hubert Kirch and Horst Röhrig 18. Determination and Detection of Reactive Oxygen Species (ROS), Lipid Peroxidation, and Electrolyte Leakage in Plants Niranjani Jambunathan Part IV: Osmotic Adjustment and Ion Measurements 19. Quantification of Water Stress-Induced Osmotic Adjustment and Proline Accumulation for Arabidopsis thaliana Molecular Genetic Studies Paul E. Verslues 20. Methods for Determination of Proline in Plants Edit Ábrahám, Cecile Hourton-Cabassa, László Erdei, and László Szabados 21. A New Method for Accurately Measuring Delta1-Pyrroline-5-Carboxylate Synthetase Activity Elodie Parre, Jacques de Virville, Françoise Cochet, Anne-Sophie Leprince, Luc Richard, Delphine Lefebvre-De Vos, Mohamed Ali Ghars, Marianne

A number of abiotic factors such as drought, salinity, extreme temperatures, low or high light intensity, and deficiency or toxic levels of nutrients have huge impacts on crop productivity, and a furthering of our understanding of the molecular, biochemical, and physiological basis of stress tolerance has been widely recognized as critical.  InPlant Stress Tolerance: Methods and Protocols, expert researchers cover the most important widely-used techniques, including cutting-edge strategies, in a manner that ensures effective results.  Beginning with reviews on dehydration, salinity, and cold tolerance as well as on oxidative stress, the volume then continues with methods involving topics such as describing the identification of stress-regulated genes, proteins, and microRNAs using diverse approaches, measurement of osmotic adjustment, proline levels, enzymes involved in proline metabolism, and sugars as well as determination of ROS levels, lipid peroxidation, ion leakage, and the enzymes involved in ROS detoxification. Written in the highly successfulMethods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Comprehensive and up-to-date,Plant Stress Tolerance: Methods and Protocolsprovides a wide range of easy-to-follow protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.